Wayne State University

BioPhysical lab

The goal of the BioPhysical Laboratory is to facilitate the integration of biomarkers into faculty and student research and scholarship through the availability of research laboratory space and equipment, provision of services, consultation, and training. The specific objectives of the lab are:

  • To provide core laboratory resources for funded faculty and student research that includes storing and managing biologic samples for downstream applications, such as genotyping, other molecular assays, or biochemical assays.
  • To provide training experiences for undergraduate and graduate students with an interest in the impact of biologic factors on phenomena for which nurses intervene.

Resources

The WSU CON Biophysical Laboratory is located on on the lower level of the Cohn building. The laboratory consists of a Research Participant Data Collection room, Research Participant Waiting area, and the Main Wet Laboratory.

Research Participant Data Collection Room and Waiting Area

The Research Participant Data Collection Room is a 72 sq. ft. space located next to the Main Wet Lab and is outfitted with a sink with running hot and cold water, cupboards, counter space, phlebotomy chair, and equipment to obtain blood pressure, temperature, and basic anthropometric measures. The Research Participant Waiting Area is a 49 sq. ft. space adjacent to the data collection room and separated by a door. The waiting area has comfortable seating with direct access to the hallway and restrooms.

Main Wet Laboratory

The Main Wet Laboratory occupies one room in the lower level of the Cohn Building with an overall size of approximately 1160 sq. ft. The Wet Laboratory is a state-of-the-art facility specifically designed to provide the space and equipment necessary to store and manage biological samples for downstream biochemical (e.g. radioimmunoassays [RIA], enzyme-linked immunoabsorbant assays [ELISA], and high-performance liquid chromatography [HPLC]) and molecular assays. The laboratory is completely equipped for molecular biology and protein analysis techniques, including but not limited to, a Flexar Quartenary LC Platform (Perkin Elmer, Waltham MA), 2910 TR TriCarb Liquid Scintillation Counter (Perkin Elmer), Wizard 2 Automatic Gamma Platform (Perkin Elmer), and an Aquamax Plate Washer and Versamax Plate Reader (Molecular Devices, Sunnyvale CA). Other equipment includes a Bayer DCA 2000 for HgbA1C analysis, a refrigerated tabletop centrifuge (Allegra 21R, Beckman Coulter, Pasadena CA), water baths, vortex mixers, pH meter, and electronic balance. In addition, the laboratory is equipped with a built in stainless steel sink, extensive bench and counter space, running hot and cold water, a water purification system (PureLab Option Q Water System), fume hood, radioactive hood (Mott Manufacturing, Ontario CA), bio safety cabinet, fire extinguisher, and emergency shower and eyewash. Biologic sample and reagents are stored and managed using a commercial refrigerator, a large capacity non-frost free -20 degree freezer, and a large capacity -80 degree freezer.